Dose calculator

NAD+ dose calculator

Convert any NAD+ dose into syringe units in real time, pre-filled with a 100 mg / 5 mL example.

iPhone · Free to download

Last updated: May 13, 2026

Vial (mg)

Diluent (mL)

Dose (mg)

Dose unit

Draw on a U-100 syringe

250.0 units

Volume to draw

2.500 mL

NAD+ is a coenzyme every cell uses to convert food into energy, and people inject it to push back against the natural age-related drop in NAD+ levels. Most users report it for energy, mental clarity, and recovery; researchers also study it for DNA-repair and metabolic-aging pathways. Human studies confirm injections raise blood NAD+ levels meaningfully, though long-term outcome data is still developing. This page covers reconstitution math and typical daily-or-cycle logging cadence.

How the NAD+ dose calculator works

NAD+ doses are unusually large — 50-100 mg per dose, taken weekly or on a flexible schedule. On a 100 mg vial mixed with 5 mL water (20 mg/mL), a 50 mg dose draws 250 units. NAD+ is the highest-volume peptide most personal-tracking users encounter.

The formula is volume in mL equals dose mg divided by concentration mg/mL, then volume times one hundred to get units on a U-100 insulin syringe. With a 20 mg/mL NAD+ solution and a 50 mg dose, the draw is 2.50 mL or about 250 units. Type any other dose and the unit count updates in real time — no spreadsheets, no guesswork.

Inputs that genuinely matter: concentration (which only changes when you reconstitute a new vial) and dose mass. Syringe type matters too, but only because U-100 vs U-40 changes the multiplier — almost every modern insulin syringe is U-100, which is why the math defaults to that. Edge cases worth flagging: switching from mcg to mg without checking the input unit, or carrying yesterday's unit count over to a new vial that was reconstituted with a different volume of BAC water.

Worked example

Walking one NAD+ dose through the math

The vial holds 100 mg of NAD+, mixed into 5 mL of bacteriostatic water — concentration 20.00 mg/mL.
Your 50 mg dose ÷ 20.00 mg/mL = 2.500 mL of solution to pull.
Multiply by 100 (because U-100 means 100 units per mL): 2.500 × 100 = 250 units.
Double the dose to 100 mg and the unit count doubles to 500 — the relationship is linear at a fixed concentration.
Change the diluent volume and every one of these numbers moves; change the dose alone and only the last one does.

NAD+ titration ladder at this concentration

What different NAD+ dose steps draw on a U-100 insulin syringe at the example 20.00 mg/mL concentration.

Dose (mg)	Volume (mL)	Units (U-100)
25	1.250	125
50	2.500	250
100	5.000	500
200	10.000	1000

Doubling the NAD+ dose doubles the unit count. Halving it halves the count. Step-ups under 5 units are hard to read accurately — re-reconstitute with more water if your titration hits that range.

Scenarios people actually run into

Three things that come up logging NAD+

You're sitting at the 50 mg NAD+ step and your prescriber bumps you up. The new dose is double — 500 units instead of 250. Same vial, same syringe, twice the volume on the line.
Your fingers reach for the syringe and the unit count from last week is still in your head. Half the time that number is fine; the other half, the vial changed and the right answer moved. The calculator is the second pair of eyes.
You skipped a week. NAD+ cadence is 1 dose per week, and doubling up to "catch up" almost never reads how people expect — log the skip, then log the next normal dose.

Same-category neighbor

NAD+ next to MOTS-c

Both sit in the Other bucket — here's the dose math side by side on each one's example vial.

	NAD+	MOTS-c
Example dose	50 mg	5 mg
Concentration	20.00 mg/mL	5.00 mg/mL
Units to draw	250	100

Want the full breakdown? MOTS-c reference →

How NAD+ dosing is tracked

Cadence and dose magnitude vary so much between users that recording the cadence explicitly in each log entry is essential. Without it, retrospective trend analysis is unreliable.

Many NAD+ users alternate between intensive loading periods and lower-frequency maintenance. Recording the transition between phases — the same way it is done for Melanotan-2 — keeps the timeline auditable.

Research protocols for subcutaneous NAD+ administration sometimes describe distinct phases for loading and maintenance. A loading phase might involve a higher frequency of administration, such as daily doses over a period of 5 to 14 days. The objective of such a phase in a research context is to rapidly alter the systemic concentration of the molecule. Following this initial period, the protocol might shift to a maintenance phase, characterized by a reduced frequency, such as a single administration per week. This two-phase structure requires diligent scheduling and tracking to accurately document the shift in dose timing and to monitor observations across both distinct periods of the protocol.

Common NAD+ dose-calculation mistakes

Trying to fit a 50 mg dose into a single insulin-syringe draw without re-running the math.
Miscalculating the dose volume and not planning for the need for multiple insulin syringes or a single larger syringe to administer the full calculated amount.
Confusing the dosing frequency and amount from a loading phase with that of a long-term maintenance phase when scheduling and documenting protocol adherence.

Frequently asked questions about NAD+ dose calculator

How does the NAD+ dose calculator turn mg into syringe units?

It runs two divisions in sequence. First it computes concentration (vial mg ÷ diluent mL) — for the example here that's 100 ÷ 5 = 20.00 mg/mL. Then it divides your dose by that concentration to get volume in mL, and multiplies by 100 to convert volume into U-100 syringe units. The output updates as you type so you can sanity-check before drawing. NAD+ injections often cause flushing or warmth — slow draws and slow injection sites help.

Does the NAD+ dose calculator know which syringe I'm using?

It assumes a U-100 insulin syringe — the most common type for sub-cutaneous peptide injections. U-100 means 100 units per mL. If you're using a U-40 syringe (rare outside veterinary contexts) the unit count is wrong by a factor of 2.5. Tuberculin syringes read in mL directly, so on those just use the volume figure. NAD+ injections often cause flushing or warmth — slow draws and slow injection sites help.

Why does the same NAD+ dose pull a different unit count today than last week?

Because either the vial or the diluent volume changed. Concentration depends on both. A 5 mg vial reconstituted with 1 mL is twice as concentrated as the same vial with 2 mL — same dose, half the units. Whenever you open a fresh vial, run the dose math again rather than carrying the prior count over. NAD+ injections often cause flushing or warmth — slow draws and slow injection sites help.

What if my NAD+ dose lands at fewer than 5 units?

That's the calculator telling you the current vial is too concentrated for the dose you want. Five units on a U-100 syringe is hard to read accurately — the markings get tight. Reconstitute the next vial with more bacteriostatic water (commonly 2 mL instead of 1 mL) so each dose covers a larger, cleaner volume. NAD+ injections often cause flushing or warmth — slow draws and slow injection sites help.

Related on Peptide Pilot

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