Calculator

NAD+ reconstitution calculator

Pre-filled with an illustrative 100 mg vial and 5 mL of bacteriostatic water. Tweak any input — the math updates instantly.

iPhone · Free to download

Last updated: May 13, 2026

Peptide in vial (mg)

Bacteriostatic water (mL)

Desired dose (mg)

Dose unit

Concentration

20.00 mg/mL

Draw (units)

250.0

Draw (mL)

2.500

Doses / vial

• Draw exceeds a single 100-unit syringe — consider more diluent or a larger syringe.

NAD+ is a coenzyme every cell uses to convert food into energy, and people inject it to push back against the natural age-related drop in NAD+ levels. Most users report it for energy, mental clarity, and recovery; researchers also study it for DNA-repair and metabolic-aging pathways. Human studies confirm injections raise blood NAD+ levels meaningfully, though long-term outcome data is still developing. This page covers reconstitution math and typical daily-or-cycle logging cadence. The calculator above is pre-filled so you can see how the math plays out for a typical NAD+ vial.

How the NAD+ reconstitution calculator works

A 100 mg NAD+ vial mixed with 5 mL of bacteriostatic water gives 20 mg/mL. A 50 mg dose pulls 2.5 mL — that's 250 units, which exceeds a single U-100 insulin syringe and usually splits across two 1 mL syringes.

In the worked example below, a 100 mg vial of NAD+ reconstituted with 5 mL of BAC water produces a concentration of 20 mg/mL. To draw the example dose of 50 mg from that vial you pull 2.50 mL — about 250 units on a standard insulin syringe. Change any input and the rest updates instantly so you can pre-plan a vial before you ever touch a needle.

Vial size, diluent volume, and dose are the three inputs that genuinely change the answer. Doses-per-vial is a derived output — it's the vial mg divided by the dose mg, rounded down. The most common edge case is a tiny dose: at very high concentration, a 0.1 mL draw is only a few units on the syringe, which is hard to read accurately. If your unit count drops below five, consider reconstituting the next vial with more BAC water so each dose covers a larger volume.

Because NAD+ vials are large, diluent volumes are also typically larger than for peptides. The illustrative example assumes a 100 mg vial reconstituted with 5 mL of bacteriostatic water — concentration of 20 mg per mL. A 50 mg illustrative dose is 2.5 mL or 250 units, which is split across multiple insulin-syringe draws or delivered with a larger syringe.

A unique consideration when planning for subcutaneous NAD+ administration is the large volume of fluid typically required per dose. Based on a common reconstitution scenario, a 100 mg vial reconstituted with 5 mL of diluent results in a concentration of 20 mg/mL. To draw an illustrative dose of 50 mg from this solution, one would need to calculate a total volume of 2.5 mL. This volume, equal to 250 units on a standard U-100 insulin syringe, exceeds the capacity of a single 1 mL (100-unit) syringe. Consequently, users must plan to either use multiple insulin syringes to draw the full volume or utilize a single, larger sterile syringe (e.g., a 3 mL or 5 mL syringe) to accommodate the entire dose in one draw.

Worked example

A worked NAD+ reconstitution, step by step

Start with the vial: 100 mg of NAD+ sitting in dry powder.
Inject 5 mL of bacteriostatic water down the inside wall — don't shoot it straight at the powder.
Concentration locks in at 100 ÷ 5 = 20.00 mg/mL for the entire life of the vial.
A 50 mg dose becomes 2.500 mL of liquid, which reads as 250 units on a U-100 syringe.
That vial has 2 clean draws in it before a partial dose at the bottom forces a new vial.

NAD+ BAC water choices for this vial

The same 100 mg NAD+ vial mixed with three different bacteriostatic water volumes. Doses-per-vial stays constant; the syringe unit count changes.

BAC water (mL)	Concentration (mg/mL)	Units for 50 mg dose
1	100.00	50
2	50.00	100
3	33.33	150

Lower BAC water volume concentrates the NAD+ solution and shrinks the unit count per dose. Higher volume spreads the dose into a more readable unit range.

Scenarios people actually run into

Three things that come up logging NAD+

Fresh 100 mg vial, no time to look things up. 5 mL of bacteriostatic water down the inside wall, swirl for a minute, write the date on the cap, done — concentration is now 20.00 mg/mL for the next 2-ish weeks.
Your previous vial was reconstituted differently. Don't trust muscle memory on the unit count — the new vial's concentration is the only number that drives this draw.
Powder didn't fully dissolve after the swirl. Wait the full five minutes before assuming anything is wrong; NAD+ is slower to dissolve than the cleanest GLP-1s, and shaking the vial is the most common way to wreck a fresh reconstitution.

Same-category neighbor

NAD+ next to Epithalon

Both sit in the Other bucket — here's the reconstitution math side by side on each one's example vial.

	NAD+	Epithalon
Vial	100 mg	10 mg
BAC water	5 mL	2 mL
Concentration	20.00 mg/mL	5.00 mg/mL

Want the full breakdown? Epithalon reference →

Reconstitution notes for NAD+

Common NAD+ reconstitution mistakes

Reusing a unit count from a previous vial without re-checking diluent volume.
Not writing the reconstitution date on the vial.
Letting reconstituted NAD+ warm to room temperature on travel days.

Frequently asked questions about NAD+ reconstitution

How much bacteriostatic water should I use for a NAD+ vial?

There's no single right answer — the diluent volume is the variable you control. With this 100 mg NAD+ vial, 2 mL is a common starting point because it produces 20.00 mg/mL, which usually puts a typical dose in a comfortable 10–30 unit range on a U-100 syringe. More water = cleaner unit counts but slightly fewer doses per vial. Less water = more doses per vial but harder-to-read syringe markings. NAD+ unit counts exceed a single U-100 syringe — most users measure in mL with a 3 mL or 5 mL syringe instead.

What's the difference between bacteriostatic water and sterile water?

Bacteriostatic (BAC) water contains 0.9 % benzyl alcohol as a preservative, which keeps the reconstituted vial usable for several weeks. Sterile water has no preservative — it's intended for single use, after which the vial should be discarded. For NAD+ vials that get drawn from multiple times, BAC water is the standard choice. NAD+ unit counts exceed a single U-100 syringe — most users measure in mL with a 3 mL or 5 mL syringe instead.

Can I shake the NAD+ vial after adding water?

Don't shake it — peptides are protein-like molecules and aggressive agitation can break them. After injecting BAC water down the inner wall of the vial, swirl gently or invert the vial a few times. It should clear within a minute or two. Cloudy solution after 5 minutes of gentle swirling is a sign the powder is degraded. NAD+ unit counts exceed a single U-100 syringe — most users measure in mL with a 3 mL or 5 mL syringe instead.

How long does a reconstituted NAD+ vial stay usable?

Most lyophilized peptides reconstituted with BAC water are typically used within 4–6 weeks of refrigerated storage. The peptide itself starts to lose potency over time, and the BAC water's preservative window has limits. Writing the reconstitution date on the vial is the easiest guard against using one past that window. NAD+ unit counts exceed a single U-100 syringe — most users measure in mL with a 3 mL or 5 mL syringe instead.

Related on Peptide Pilot

Save NAD+ vials in the app

Peptide Pilot stores every vial once and derives every subsequent dose, draw, and refill reminder from those numbers automatically.

iPhone · Free · No credit card