Dose calculator

AOD-9604 dose calculator

Convert any AOD-9604 dose into syringe units in real time, pre-filled with a 5 mg / 2 mL example.

Draw on a U-100 syringe

12.0 units

Volume to draw

0.120 mL

AOD-9604 is a small, modified piece of human growth hormone (hGH) that people explore for its effects on fat metabolism. Unlike full-length growth hormone, it was developed specifically to target fat cells without affecting blood sugar or causing growth. Early studies, including a Phase 2b trial in obese adults, reported that it influenced fat loss, though research is still considered preliminary. This page covers what AOD-9604 is, how it’s thought to work, and the ways people track its use in the Peptide Pilot app.

How the AOD-9604 dose calculator works

AOD-9604 doses sit at 250-500 mcg daily for fat-metabolism research protocols. On a 5 mg vial mixed with 2 mL water (2.5 mg/mL), a 300 mcg dose draws 12 units. Daily cadence with small unit counts — readable but not generous.

The formula is volume in mL equals dose mg divided by concentration mg/mL, then volume times one hundred to get units on a U-100 insulin syringe. With a 2.5 mg/mL AOD-9604 solution and a 0.3 mg dose, the draw is 0.12 mL or about 12 units. Type any other dose and the unit count updates in real time — no spreadsheets, no guesswork.

Inputs that genuinely matter: concentration (which only changes when you reconstitute a new vial) and dose mass. Syringe type matters too, but only because U-100 vs U-40 changes the multiplier — almost every modern insulin syringe is U-100, which is why the math defaults to that. Edge cases worth flagging: switching from mcg to mg without checking the input unit, or carrying yesterday's unit count over to a new vial that was reconstituted with a different volume of BAC water.

Figuring out the right amount of liquid to draw for your dose can feel like a tricky math problem, but it doesn’t have to be. The Dose Volume calculator on this page is designed specifically to solve this for you. Your AOD-9604 protocol might call for a precise dose, like 0.3mg. The challenge is that your syringe doesn’t measure in milligrams; it measures in milliliters or units. This calculator acts as the perfect translator between the two, ensuring you get the accuracy you need for consistent tracking.

Let’s use our example. You’ve mixed your 5mg vial of AOD-9604 with 2mL of water. You simply input these numbers into the calculator, along with your desired 0.3mg dose. The tool instantly tells you the exact amount to draw up: 12 Units. There’s no need to scribble calculations on a piece of paper or risk making a small error that could throw off your entire protocol. Consistency is a key factor when evaluating how something works for you, and that consistency starts with an accurate dose every single time.

Think of this calculator as your partner for every administration. Whether you’re just starting with a titration dose or you’re on a steady daily protocol, this tool adapts to your needs. If you decide to adjust your dose from 0.3mg to 0.35mg, you can quickly find the new unit amount. By removing the guesswork, the dosing calculator lets you focus less on the math and more on the other important parts of your protocol, like timing, diet, and logging the effects you observe.

Worked example

Walking one AOD-9604 dose through the math

  1. The vial holds 5 mg of AOD-9604, mixed into 2 mL of bacteriostatic water — concentration 2.50 mg/mL.
  2. Your 0.3 mg dose ÷ 2.50 mg/mL = 0.120 mL of solution to pull.
  3. Multiply by 100 (because U-100 means 100 units per mL): 0.120 × 100 = 12 units.
  4. Double the dose to 0.6 mg and the unit count doubles to 24 — the relationship is linear at a fixed concentration.
  5. Change the diluent volume and every one of these numbers moves; change the dose alone and only the last one does.

AOD-9604 titration ladder at this concentration

What different AOD-9604 dose steps draw on a U-100 insulin syringe at the example 2.50 mg/mL concentration.

Dose (mg)Volume (mL)Units (U-100)
0.150.0606
0.30.12012
0.60.24024
1.20.48048

Doubling the AOD-9604 dose doubles the unit count. Halving it halves the count. Step-ups under 5 units are hard to read accurately — re-reconstitute with more water if your titration hits that range.

Scenarios people actually run into

Three things that come up logging AOD-9604

  • You're sitting at the 0.3 mg AOD-9604 step and your prescriber bumps you up. The new dose is double — 24 units instead of 12. Same vial, same syringe, twice the volume on the line.
  • Your fingers reach for the syringe and the unit count from last week is still in your head. Half the time that number is fine; the other half, the vial changed and the right answer moved. The calculator is the second pair of eyes.
  • You skipped a week. AOD-9604 cadence is 7 doses per week, and doubling up to "catch up" almost never reads how people expect — log the skip, then log the next normal dose.

Same-category neighbor

AOD-9604 next to Tirzepatide

Both sit in the Fat loss bucket — here's the dose math side by side on each one's example vial.

AOD-9604Tirzepatide
Example dose0.3 mg2.5 mg
Concentration2.50 mg/mL5.00 mg/mL
Units to draw1250

Want the full breakdown? Tirzepatide reference →

How AOD-9604 dosing is tracked

The most common protocol for AOD-9604 involves a single daily administration. Users typically track a dose ranging from 250 mcg to 350 mcg (or 0.25mg to 0.35mg). This dose is almost always taken on an empty stomach, which is considered a critical part of the protocol. The most frequent timing is first thing in the morning, at least 30-60 minutes before the first meal of the day. The rationale behind this timing is to allow the peptide to work in a "fasted state" when insulin levels are low. High levels of insulin, which are present after a meal, can interfere with lipolysis and may blunt the potential effects of the peptide.

After the morning injection, users often continue to fast for a period of time, with some protocols suggesting waiting up to three hours before eating. This is to provide the longest possible window for the released fatty acids to be used by the body for energy. Taking AOD-9604 and then immediately eating a carbohydrate-rich meal would be counterproductive, as the body would likely just use the energy from the food and re-store the fatty acids that the peptide helped release. The effectiveness of the protocol is often seen as being tightly linked to disciplined timing around meals and an overall diet that supports the use of fat for fuel.

In terms of cycling, there is no single established rule, and practices vary. Some users track AOD-9604 continuously for several months, while others prefer a more cyclical approach, such as using it for eight to twelve weeks followed by a break of similar length. Another common strategy is to use it five days a week with a two-day break over the weekend. This "5 on, 2 off" pattern is thought by some to help maintain the body’s sensitivity to the peptide over longer periods. Titration is not always necessary with AOD-9604 as it is generally well-tolerated, but some individuals may start with a lower dose (e.g., 150-200 mcg) for the first week to assess their personal response before moving up to their target dose.

Common AOD-9604 dose-calculation mistakes

  • Miscalculating the dose on the syringe, leading to inconsistent and unreliable tracking.
  • Dosing right after a carbohydrate-heavy meal, which can hinder its fat-releasing action.

Frequently asked questions about AOD-9604 dose calculator

How does the AOD-9604 dose calculator turn mg into syringe units?
It runs two divisions in sequence. First it computes concentration (vial mg ÷ diluent mL) — for the example here that's 5 ÷ 2 = 2.50 mg/mL. Then it divides your dose by that concentration to get volume in mL, and multiplies by 100 to convert volume into U-100 syringe units. The output updates as you type so you can sanity-check before drawing. AOD-9604 is a 16-amino-acid fragment of hGH — the dose ranges are similar to GH secretagogues but the mechanism is different.
Does the AOD-9604 dose calculator know which syringe I'm using?
It assumes a U-100 insulin syringe — the most common type for sub-cutaneous peptide injections. U-100 means 100 units per mL. If you're using a U-40 syringe (rare outside veterinary contexts) the unit count is wrong by a factor of 2.5. Tuberculin syringes read in mL directly, so on those just use the volume figure. AOD-9604 is a 16-amino-acid fragment of hGH — the dose ranges are similar to GH secretagogues but the mechanism is different.
Why does the same AOD-9604 dose pull a different unit count today than last week?
Because either the vial or the diluent volume changed. Concentration depends on both. A 5 mg vial reconstituted with 1 mL is twice as concentrated as the same vial with 2 mL — same dose, half the units. Whenever you open a fresh vial, run the dose math again rather than carrying the prior count over. AOD-9604 is a 16-amino-acid fragment of hGH — the dose ranges are similar to GH secretagogues but the mechanism is different.
What if my AOD-9604 dose lands at fewer than 5 units?
That's the calculator telling you the current vial is too concentrated for the dose you want. Five units on a U-100 syringe is hard to read accurately — the markings get tight. Reconstitute the next vial with more bacteriostatic water (commonly 2 mL instead of 1 mL) so each dose covers a larger, cleaner volume. AOD-9604 is a 16-amino-acid fragment of hGH — the dose ranges are similar to GH secretagogues but the mechanism is different.

Related on Peptide Pilot

Log every AOD-9604 dose in the app

Download on the App StoreiPhone · Free · No credit card