MOTS-c reconstitution calculator

A 10 mg MOTS-c vial mixed with 2 mL of bacteriostatic water gives 5 mg/mL. A 5 mg dose pulls 1 mL or 100 units. The vial covers 2 doses, so 3-times-weekly cadence burns through one vial in under a week.

In the worked example below, a 10 mg vial of MOTS-c reconstituted with 2 mL of BAC water produces a concentration of 5 mg/mL. To draw the example dose of 5 mg from that vial you pull 1.00 mL — about 100 units on a standard insulin syringe. Change any input and the rest updates instantly so you can pre-plan a vial before you ever touch a needle.

Vial size, diluent volume, and dose are the three inputs that genuinely change the answer. Doses-per-vial is a derived output — it's the vial mg divided by the dose mg, rounded down. The most common edge case is a tiny dose: at very high concentration, a 0.1 mL draw is only a few units on the syringe, which is hard to read accurately. If your unit count drops below five, consider reconstituting the next vial with more BAC water so each dose covers a larger volume.

The process of reconstitution requires a precise calculation to ensure accurate dosing. For a standard 10 mg vial of MOTS-c, adding 2 mL of bacteriostatic water will dissolve the lyophilized powder and yield a final concentration of 5 mg per mL. To administer a 5 mg dose from this specific solution, one must draw exactly 1 mL of liquid. On a U-100 insulin syringe, where the 1 mL total capacity is marked as 100 units, this volume corresponds to a full draw of 100 units.

The volume of diluent used is a key variable that determines the final injection volume. A 5 mg dose, as in the example, is a substantial amount, and using 2 mL of diluent results in a large 1 mL injection. Some individuals may document using a smaller diluent volume, such as 1 mL, to create a more concentrated solution (10 mg/mL). In that case, the same 5 mg dose would require only 0.5 mL (50 units), a significantly smaller volume to inject. The calculator on this site allows for planning and documenting these variables to maintain a consistent and auditable record.

A key point to document when reconstituting MOTS-c is its commonly studied dose range. Published studies frequently observe doses in the milligram range (e.g., 5 mg), substantially higher than many peptides dosed in micrograms. This distinction necessitates careful calculation. For example, a 10 mg vial reconstituted with 2 mL results in a 5 mg/mL solution. A 5 mg illustrative dose is then 1 mL, or 100 units on a U-100 syringe. Accurately calculating this concentration is critical for correct documentation.