Calculator

Epithalon reconstitution calculator

Pre-filled with an illustrative 10 mg vial and 2 mL of bacteriostatic water. Tweak any input — the math updates instantly.

iPhone · Free to download

Last updated: May 13, 2026

Peptide in vial (mg)

Bacteriostatic water (mL)

Desired dose (mg)

Dose unit

Concentration

5.00 mg/mL

Draw (units)

100.0

Draw (mL)

1.000

Doses / vial

Epithalon is a short four-amino-acid peptide people use in cycles, usually for sleep quality and as a longevity-adjacent experiment. The interest comes from research suggesting it can lengthen telomeres — the protective caps on the ends of chromosomes — and influence the pineal gland's melatonin rhythm. The original Russian trials reported telomere-length increases and improved sleep markers, but independent replication is limited. This page covers reconstitution math and how people log a typical 10–20 day cycle. The calculator above is pre-filled so you can see how the math plays out for a typical Epithalon vial.

How the Epithalon reconstitution calculator works

A 10 mg Epithalon vial mixed with 2 mL of bacteriostatic water gives 5 mg/mL. A 5 mg dose pulls 1 mL or 100 units — exactly one full U-100 syringe. The vial covers 2 doses, so cycles run 1 vial every 2 days.

In the worked example below, a 10 mg vial of Epithalon reconstituted with 2 mL of BAC water produces a concentration of 5 mg/mL. To draw the example dose of 5 mg from that vial you pull 1.00 mL — about 100 units on a standard insulin syringe. Change any input and the rest updates instantly so you can pre-plan a vial before you ever touch a needle.

Vial size, diluent volume, and dose are the three inputs that genuinely change the answer. Doses-per-vial is a derived output — it's the vial mg divided by the dose mg, rounded down. The most common edge case is a tiny dose: at very high concentration, a 0.1 mL draw is only a few units on the syringe, which is hard to read accurately. If your unit count drops below five, consider reconstituting the next vial with more BAC water so each dose covers a larger volume.

To accurately calculate dose volume, one must first determine the final concentration of the reconstituted solution. For instance, if a 10 mg vial of lyophilized Epithalon is reconstituted with 2 mL of bacteriostatic water, the resulting concentration is 5 mg per mL. To prepare an example dose of 5 mg, the required volume to draw would be 1.0 mL. On a standard U-100 insulin syringe, this volume converts to exactly 100 units.

A 100-unit draw represents the full capacity of a 1 mL insulin syringe, which can be an awkward volume to administer precisely and without leaving any peptide behind in the syringe hub. To create a more manageable injection volume, many prefer to increase the amount of diluent used. Reconstituting the same 10 mg vial with 4 mL of bacteriostatic water, for example, would create a concentration of 2.5 mg/mL; a 5 mg dose would then require a 2.0 mL draw which is too large. A better alternative is often to use less than 2mL, for example 1mL which would result in a 0.5mL or 50 unit draw. The choice of diluent volume is therefore a practical workflow decision, balancing ease of measurement against the number of injections obtainable from a single vial.

Worked example

A worked Epithalon reconstitution, step by step

Start with the vial: 10 mg of Epithalon sitting in dry powder.
Inject 2 mL of bacteriostatic water down the inside wall — don't shoot it straight at the powder.
Concentration locks in at 10 ÷ 2 = 5.00 mg/mL for the entire life of the vial.
A 5 mg dose becomes 1.000 mL of liquid, which reads as 100 units on a U-100 syringe.
That vial has 2 clean draws in it before a partial dose at the bottom forces a new vial.

Epithalon BAC water choices for this vial

The same 10 mg Epithalon vial mixed with three different bacteriostatic water volumes. Doses-per-vial stays constant; the syringe unit count changes.

BAC water (mL)	Concentration (mg/mL)	Units for 5 mg dose
1	10.00	50
2	5.00	100
3	3.33	150

Lower BAC water volume concentrates the Epithalon solution and shrinks the unit count per dose. Higher volume spreads the dose into a more readable unit range.

Scenarios people actually run into

Three things that come up logging Epithalon

Fresh 10 mg vial, no time to look things up. 2 mL of bacteriostatic water down the inside wall, swirl for a minute, write the date on the cap, done — concentration is now 5.00 mg/mL for the next 0-ish weeks.
Your previous vial was reconstituted differently. Don't trust muscle memory on the unit count — the new vial's concentration is the only number that drives this draw.
Powder didn't fully dissolve after the swirl. Wait the full five minutes before assuming anything is wrong; Epithalon is slower to dissolve than the cleanest GLP-1s, and shaking the vial is the most common way to wreck a fresh reconstitution.

Same-category neighbor

Epithalon next to MOTS-c

Both sit in the Other bucket — here's the reconstitution math side by side on each one's example vial.

	Epithalon	MOTS-c
Vial	10 mg	10 mg
BAC water	2 mL	2 mL
Concentration	5.00 mg/mL	5.00 mg/mL

Want the full breakdown? MOTS-c reference →

Reconstitution notes for Epithalon

Common Epithalon reconstitution mistakes

Reconstituting a 10 mg vial with 2 mL of diluent and not anticipating that a 5 mg dose requires drawing the full 100-unit capacity of a 1 mL syringe.

Frequently asked questions about Epithalon reconstitution

How much bacteriostatic water should I use for a Epithalon vial?

There's no single right answer — the diluent volume is the variable you control. With this 10 mg Epithalon vial, 2 mL is a common starting point because it produces 5.00 mg/mL, which usually puts a typical dose in a comfortable 10–30 unit range on a U-100 syringe. More water = cleaner unit counts but slightly fewer doses per vial. Less water = more doses per vial but harder-to-read syringe markings. Epithalon's larger doses mean unit counts often hit 100+ — split into two 50-unit draws for cleaner injections.

What's the difference between bacteriostatic water and sterile water?

Bacteriostatic (BAC) water contains 0.9 % benzyl alcohol as a preservative, which keeps the reconstituted vial usable for several weeks. Sterile water has no preservative — it's intended for single use, after which the vial should be discarded. For Epithalon vials that get drawn from multiple times, BAC water is the standard choice. Epithalon's larger doses mean unit counts often hit 100+ — split into two 50-unit draws for cleaner injections.

Can I shake the Epithalon vial after adding water?

Don't shake it — peptides are protein-like molecules and aggressive agitation can break them. After injecting BAC water down the inner wall of the vial, swirl gently or invert the vial a few times. It should clear within a minute or two. Cloudy solution after 5 minutes of gentle swirling is a sign the powder is degraded. Epithalon's larger doses mean unit counts often hit 100+ — split into two 50-unit draws for cleaner injections.

How long does a reconstituted Epithalon vial stay usable?

Most lyophilized peptides reconstituted with BAC water are typically used within 4–6 weeks of refrigerated storage. The peptide itself starts to lose potency over time, and the BAC water's preservative window has limits. Writing the reconstitution date on the vial is the easiest guard against using one past that window. Epithalon's larger doses mean unit counts often hit 100+ — split into two 50-unit draws for cleaner injections.

Related on Peptide Pilot

Save Epithalon vials in the app

Peptide Pilot stores every vial once and derives every subsequent dose, draw, and refill reminder from those numbers automatically.

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