Calculator

Tesamorelin reconstitution calculator

Pre-filled with an illustrative 5 mg vial and 2 mL of bacteriostatic water. Tweak any input — the math updates instantly.

Concentration

2.50 mg/mL

Draw (units)

40.0

Draw (mL)

0.400

Doses / vial

5

Tesamorelin is a daily injection people use specifically to reduce stubborn deep belly fat (visceral adipose tissue). It's an analog of growth hormone-releasing hormone (GHRH) that prompts the pituitary to release more of the body's own GH. In FDA trials for HIV-related lipodystrophy, daily 2 mg injections reduced visceral fat by about 15–18% over 26 weeks. This page covers reconstitution math and daily dose logging. The calculator above is pre-filled so you can see how the math plays out for a typical Tesamorelin vial.

How the Tesamorelin reconstitution calculator works

A 5 mg tesamorelin vial mixed with 2 mL of bacteriostatic water gives 2.5 mg/mL. A 1 mg dose pulls 0.4 mL or 40 units — readable, repeatable. With daily injection, the vial covers 5 doses, so weekly refill cadence is the planning rhythm.

In the worked example below, a 5 mg vial of Tesamorelin reconstituted with 2 mL of BAC water produces a concentration of 2.5 mg/mL. To draw the example dose of 1 mg from that vial you pull 0.40 mL — about 40 units on a standard insulin syringe. Change any input and the rest updates instantly so you can pre-plan a vial before you ever touch a needle.

Vial size, diluent volume, and dose are the three inputs that genuinely change the answer. Doses-per-vial is a derived output — it's the vial mg divided by the dose mg, rounded down. The most common edge case is a tiny dose: at very high concentration, a 0.1 mL draw is only a few units on the syringe, which is hard to read accurately. If your unit count drops below five, consider reconstituting the next vial with more BAC water so each dose covers a larger volume.

The calculation to determine the correct syringe volume for a dose begins with the vial's total peptide content and the chosen volume of diluent. To illustrate with a common scenario, if a 5 mg vial of Tesamorelin is reconstituted using 2 mL of bacteriostatic water, the final concentration of the solution becomes 2.5 mg per mL. To draw a target dose of 1 mg, one would need to calculate the corresponding volume (1 mg divided by 2.5 mg/mL equals 0.40 mL), which converts precisely to 40 units on a U-100 insulin syringe.

Given the larger per-dose magnitude of Tesamorelin, the choice of diluent volume is a more significant planning variable than it is for microgram-dosed peptides. Using a smaller volume of bacteriostatic water (e.g., 1 mL in a 5 mg vial) will yield a highly concentrated solution, reducing the physical volume of the injection but potentially making very small dose adjustments difficult to measure. Conversely, using a larger diluent volume like 2 mL creates a less concentrated solution and a larger injection volume (e.g., 40 units for a 1 mg dose), which may improve measurement precision at the expense of requiring more careful injection site management.

When documenting the reconstitution process for tesamorelin, it is important to note the distinction between research-grade preparations and the pharmaceutical version, Egrifta. The latter is supplied in a kit with a specific volume of sterile water for injection, establishing a standardized final concentration. For individuals documenting personal research with a lyophilized powder vial, it is crucial to log the exact type and volume of the diluent used, such as bacteriostatic water. Recording this information ensures that all subsequent dose calculations logged in the platform are accurate and that the concentration can be audited against the planned protocol.

Worked example

A worked Tesamorelin reconstitution, step by step

  1. Start with the vial: 5 mg of Tesamorelin sitting in dry powder.
  2. Inject 2 mL of bacteriostatic water down the inside wall — don't shoot it straight at the powder.
  3. Concentration locks in at 5 ÷ 2 = 2.50 mg/mL for the entire life of the vial.
  4. A 1 mg dose becomes 0.400 mL of liquid, which reads as 40 units on a U-100 syringe.
  5. That vial has 5 clean draws in it before a partial dose at the bottom forces a new vial.

Tesamorelin BAC water choices for this vial

The same 5 mg Tesamorelin vial mixed with three different bacteriostatic water volumes. Doses-per-vial stays constant; the syringe unit count changes.

BAC water (mL)Concentration (mg/mL)Units for 1 mg dose
15.0020
22.5040
31.6760

Lower BAC water volume concentrates the Tesamorelin solution and shrinks the unit count per dose. Higher volume spreads the dose into a more readable unit range.

Scenarios people actually run into

Three things that come up logging Tesamorelin

  • Fresh 5 mg vial, no time to look things up. 2 mL of bacteriostatic water down the inside wall, swirl for a minute, write the date on the cap, done — concentration is now 2.50 mg/mL for the next 1-ish weeks.
  • Your previous vial was reconstituted differently. Don't trust muscle memory on the unit count — the new vial's concentration is the only number that drives this draw.
  • Powder didn't fully dissolve after the swirl. Wait the full five minutes before assuming anything is wrong; Tesamorelin is slower to dissolve than the cleanest GLP-1s, and shaking the vial is the most common way to wreck a fresh reconstitution.

Same-category neighbor

Tesamorelin next to Sermorelin

Both sit in the GH Secretagogue bucket — here's the reconstitution math side by side on each one's example vial.

TesamorelinSermorelin
Vial5 mg5 mg
BAC water2 mL2 mL
Concentration2.50 mg/mL2.50 mg/mL

Want the full breakdown? Sermorelin reference →

Reconstitution notes for Tesamorelin

The calculation to determine the correct syringe volume for a dose begins with the vial's total peptide content and the chosen volume of diluent. To illustrate with a common scenario, if a 5 mg vial of Tesamorelin is reconstituted using 2 mL of bacteriostatic water, the final concentration of the solution becomes 2.5 mg per mL. To draw a target dose of 1 mg, one would need to calculate the corresponding volume (1 mg divided by 2.5 mg/mL equals 0.40 mL), which converts precisely to 40 units on a U-100 insulin syringe.

Given the larger per-dose magnitude of Tesamorelin, the choice of diluent volume is a more significant planning variable than it is for microgram-dosed peptides. Using a smaller volume of bacteriostatic water (e.g., 1 mL in a 5 mg vial) will yield a highly concentrated solution, reducing the physical volume of the injection but potentially making very small dose adjustments difficult to measure. Conversely, using a larger diluent volume like 2 mL creates a less concentrated solution and a larger injection volume (e.g., 40 units for a 1 mg dose), which may improve measurement precision at the expense of requiring more careful injection site management.

When documenting the reconstitution process for tesamorelin, it is important to note the distinction between research-grade preparations and the pharmaceutical version, Egrifta. The latter is supplied in a kit with a specific volume of sterile water for injection, establishing a standardized final concentration. For individuals documenting personal research with a lyophilized powder vial, it is crucial to log the exact type and volume of the diluent used, such as bacteriostatic water. Recording this information ensures that all subsequent dose calculations logged in the platform are accurate and that the concentration can be audited against the planned protocol.

Common Tesamorelin reconstitution mistakes

  • Attempting to reconstitute a 5 mg vial with an excessively small diluent volume, making the large 1 mg dose difficult to measure and draw accurately.

Frequently asked questions about Tesamorelin reconstitution

How much bacteriostatic water should I use for a Tesamorelin vial?
There's no single right answer — the diluent volume is the variable you control. With this 5 mg Tesamorelin vial, 2 mL is a common starting point because it produces 2.50 mg/mL, which usually puts a typical dose in a comfortable 10–30 unit range on a U-100 syringe. More water = cleaner unit counts but slightly fewer doses per vial. Less water = more doses per vial but harder-to-read syringe markings. Tesamorelin is one of the few GH-family peptides dosed in mg — keep that distinction clear when reading dose tables.
What's the difference between bacteriostatic water and sterile water?
Bacteriostatic (BAC) water contains 0.9 % benzyl alcohol as a preservative, which keeps the reconstituted vial usable for several weeks. Sterile water has no preservative — it's intended for single use, after which the vial should be discarded. For Tesamorelin vials that get drawn from multiple times, BAC water is the standard choice. Tesamorelin is one of the few GH-family peptides dosed in mg — keep that distinction clear when reading dose tables.
Can I shake the Tesamorelin vial after adding water?
Don't shake it — peptides are protein-like molecules and aggressive agitation can break them. After injecting BAC water down the inner wall of the vial, swirl gently or invert the vial a few times. It should clear within a minute or two. Cloudy solution after 5 minutes of gentle swirling is a sign the powder is degraded. Tesamorelin is one of the few GH-family peptides dosed in mg — keep that distinction clear when reading dose tables.
How long does a reconstituted Tesamorelin vial stay usable?
Most lyophilized peptides reconstituted with BAC water are typically used within 4–6 weeks of refrigerated storage. The peptide itself starts to lose potency over time, and the BAC water's preservative window has limits. Writing the reconstitution date on the vial is the easiest guard against using one past that window. Tesamorelin is one of the few GH-family peptides dosed in mg — keep that distinction clear when reading dose tables.

Related on Peptide Pilot

Save Tesamorelin vials in the app

Peptide Pilot stores every vial once and derives every subsequent dose, draw, and refill reminder from those numbers automatically.

Download on the App StoreiPhone · Free · No credit card