Dose calculator

TB-500 dose calculator

Convert any TB-500 dose into syringe units in real time, pre-filled with a 5 mg / 2 mL example.

iPhone · Free to download

Last updated: May 13, 2026

Vial (mg)

Diluent (mL)

Dose (mg)

Dose unit

Draw on a U-100 syringe

80.0 units

Volume to draw

0.800 mL

TB-500 is a synthetic fragment of the natural protein Thymosin Beta-4 that people use to support recovery from soft-tissue and tendon injuries. It works by promoting cell migration and new blood-vessel formation at injury sites, which is what allows damaged tissue to rebuild faster. Animal studies show meaningful acceleration of wound and tendon healing; controlled human data is limited. This page covers reconstitution math and how people typically log a loading-then-maintenance schedule.

How the TB-500 dose calculator works

TB-500 doses are larger than BPC-157 — typically 2 mg to 5 mg per injection, taken twice a week. On a 5 mg vial mixed with 2 mL water (2.5 mg/mL), a 2 mg dose draws 80 units. That's most of one syringe, so readability is easy.

The formula is volume in mL equals dose mg divided by concentration mg/mL, then volume times one hundred to get units on a U-100 insulin syringe. With a 2.5 mg/mL TB-500 solution and a 2 mg dose, the draw is 0.80 mL or about 80 units. Type any other dose and the unit count updates in real time — no spreadsheets, no guesswork.

Inputs that genuinely matter: concentration (which only changes when you reconstitute a new vial) and dose mass. Syringe type matters too, but only because U-100 vs U-40 changes the multiplier — almost every modern insulin syringe is U-100, which is why the math defaults to that. Edge cases worth flagging: switching from mcg to mg without checking the input unit, or carrying yesterday's unit count over to a new vial that was reconstituted with a different volume of BAC water.

Worked example

Walking one TB-500 dose through the math

The vial holds 5 mg of TB-500, mixed into 2 mL of bacteriostatic water — concentration 2.50 mg/mL.
Your 2 mg dose ÷ 2.50 mg/mL = 0.800 mL of solution to pull.
Multiply by 100 (because U-100 means 100 units per mL): 0.800 × 100 = 80 units.
Double the dose to 4 mg and the unit count doubles to 160 — the relationship is linear at a fixed concentration.
Change the diluent volume and every one of these numbers moves; change the dose alone and only the last one does.

TB-500 titration ladder at this concentration

What different TB-500 dose steps draw on a U-100 insulin syringe at the example 2.50 mg/mL concentration.

Dose (mg)	Volume (mL)	Units (U-100)
1	0.400	40
2	0.800	80
4	1.600	160
8	3.200	320

Doubling the TB-500 dose doubles the unit count. Halving it halves the count. Step-ups under 5 units are hard to read accurately — re-reconstitute with more water if your titration hits that range.

Scenarios people actually run into

Three things that come up logging TB-500

You're sitting at the 2 mg TB-500 step and your prescriber bumps you up. The new dose is double — 160 units instead of 80. Same vial, same syringe, twice the volume on the line.
Your fingers reach for the syringe and the unit count from last week is still in your head. Half the time that number is fine; the other half, the vial changed and the right answer moved. The calculator is the second pair of eyes.
You skipped a week. TB-500 cadence is 2 doses per week, and doubling up to "catch up" almost never reads how people expect — log the skip, then log the next normal dose.

Same-category neighbor

TB-500 next to BPC-157

Both sit in the Healing bucket — here's the dose math side by side on each one's example vial.

	TB-500	BPC-157
Example dose	2 mg	0.25 mg
Concentration	2.50 mg/mL	2.50 mg/mL
Units to draw	80	10

Want the full breakdown? BPC-157 reference →

How TB-500 dosing is tracked

Logs documenting TB-500 administration most often show a subcutaneous cadence of once or twice per week, a pattern consistent with a molecule expected to have a prolonged duration of action. Some experimental designs incorporate an initial loading phase, where administration might occur several times per week for one to four weeks, before transitioning to a less frequent maintenance schedule. Due to the milligram-scale doses, a 1mL or 0.5mL U-100 insulin syringe is typically used to accurately draw the calculated volume from the reconstituted vial. Rotation of injection sites is a standard practice recorded in detailed logs to monitor for any localized skin reactions.

When planned in conjunction with a peptide requiring daily administration, like BPC-157, TB-500 is scheduled on its own rhythm within the week. A tracker might record daily BPC-157 entries while logging TB-500 doses only on Mondays and Thursdays, for example. This separation ensures that each protocol can be monitored independently without complex timing interactions. The precise time of day for a TB-500 dose is often considered less critical than for short-acting peptides, as the goal is to maintain a stable systemic concentration over many days rather than targeting a narrow post-injection activity window.

Common TB-500 dose-calculation mistakes

Using only 1 mL of diluent for a 10 mg vial and finding the resulting solution too concentrated to measure small dose adjustments precisely on a U-100 syringe.
Assuming the same unit measurement as BPC-157 when they are stacked, leading to a significant under-dose of TB-500 due to its milligram-scale dosing.
Allowing a twice-weekly schedule to drift by a day each week, altering the dosing interval from a 3-day/4-day pattern to a 4-day/5-day pattern over time.

Frequently asked questions about TB-500 dose calculator

How does the TB-500 dose calculator turn mg into syringe units?

It runs two divisions in sequence. First it computes concentration (vial mg ÷ diluent mL) — for the example here that's 5 ÷ 2 = 2.50 mg/mL. Then it divides your dose by that concentration to get volume in mL, and multiplies by 100 to convert volume into U-100 syringe units. The output updates as you type so you can sanity-check before drawing. TB-500 loading protocols often run 4-6 weeks of twice-weekly dosing, then drop to weekly maintenance.

Does the TB-500 dose calculator know which syringe I'm using?

It assumes a U-100 insulin syringe — the most common type for sub-cutaneous peptide injections. U-100 means 100 units per mL. If you're using a U-40 syringe (rare outside veterinary contexts) the unit count is wrong by a factor of 2.5. Tuberculin syringes read in mL directly, so on those just use the volume figure. TB-500 loading protocols often run 4-6 weeks of twice-weekly dosing, then drop to weekly maintenance.

Why does the same TB-500 dose pull a different unit count today than last week?

Because either the vial or the diluent volume changed. Concentration depends on both. A 5 mg vial reconstituted with 1 mL is twice as concentrated as the same vial with 2 mL — same dose, half the units. Whenever you open a fresh vial, run the dose math again rather than carrying the prior count over. TB-500 loading protocols often run 4-6 weeks of twice-weekly dosing, then drop to weekly maintenance.

What if my TB-500 dose lands at fewer than 5 units?

That's the calculator telling you the current vial is too concentrated for the dose you want. Five units on a U-100 syringe is hard to read accurately — the markings get tight. Reconstitute the next vial with more bacteriostatic water (commonly 2 mL instead of 1 mL) so each dose covers a larger, cleaner volume. TB-500 loading protocols often run 4-6 weeks of twice-weekly dosing, then drop to weekly maintenance.

Related on Peptide Pilot

Log every TB-500 dose in the app

iPhone · Free · No credit card