AOD-9604 reconstitution calculator

A 5 mg AOD-9604 vial mixed with 2 mL of bacteriostatic water gives 2.5 mg/mL. A 300 mcg dose pulls 0.12 mL or 12 units. The vial covers 16 daily doses — about 2.5 weeks at the daily cadence most fat-loss protocols use.

In the worked example below, a 5 mg vial of AOD-9604 reconstituted with 2 mL of BAC water produces a concentration of 2.5 mg/mL. To draw the example dose of 0.3 mg from that vial you pull 0.12 mL — about 12 units on a standard insulin syringe. Change any input and the rest updates instantly so you can pre-plan a vial before you ever touch a needle.

Vial size, diluent volume, and dose are the three inputs that genuinely change the answer. Doses-per-vial is a derived output — it's the vial mg divided by the dose mg, rounded down. The most common edge case is a tiny dose: at very high concentration, a 0.1 mL draw is only a few units on the syringe, which is hard to read accurately. If your unit count drops below five, consider reconstituting the next vial with more BAC water so each dose covers a larger volume.

Reconstituting AOD-9604 correctly is the first step toward consistent and accurate tracking. The peptide arrives as a delicate, freeze-dried powder that must be mixed into a liquid solution before it can be measured and administered. The standard liquid for this is bacteriostatic water, which is sterile water that contains a small amount of benzyl alcohol to prevent bacterial growth. When you are ready to mix, you’ll first want to gently tap the vial to make sure all the powder has settled at the bottom. Then, using a fresh syringe, draw up your chosen amount of bacteriostatic water—in our example, we will use 2mL.

With your 2mL of water in the syringe, you will carefully insert the needle through the rubber stopper of the AOD-9604 vial. Angle the needle so the water runs down the inside wall of the vial instead of spraying directly onto the peptide powder. This gentle technique helps prevent any potential damage to the fragile peptide molecules. Once all the water is in, you should gently swirl the vial or roll it between your hands until the powder is fully dissolved. Do not shake the vial vigorously. The final solution should be completely clear. With a 5mg vial and 2mL of water, your final concentration is 2.5mg of AOD-9604 for every 1mL of liquid.

Now, let's look at how to draw a specific dose. If your target dose is 0.3mg, you need to do a little math, which the Peptide Pilot calculator handles for you. With a concentration of 2.5mg/mL, the calculation is: (0.3mg dose / 2.5mg per mL) = 0.12mL of liquid. Since a standard insulin syringe measures volume in units (where 100 units equals 1mL), you would need to draw 12 units on the syringe to get your 0.3mg dose. Being precise in both reconstitution and dose drawing is key to a protocol’s consistency and makes the data you track in the app much more reliable.